What Is Epsilon Poly L Lysine?

What is Epsilon Poly L Lysine?

Epsilon Poly L Lysine (ε-Polylysine) is a natural food preservative and high-efficiency antimicrobial agent manufactured from a fermentation process using Streptomyces albulus under aerobic conditions. Epsilon poly l lysine (ε-Polylysine) is widely used as food preservative. It can inhibit the growth of Gram-positive and Gram-negative bacteria, yeasts, moulds, bacteria and ect.

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ε-Polylysine is also called Epsilon Polylysine, ε-Poly-L-lysine, ε Poly L Lysine, ε-PL, Natural antimicrobial preservative.

Epsilon Poly L Lysine structure

Epsilon Poly L Lysine (ε-Polylysine) is a homopolymer of L-lysine, one of the essential amino acids. It is linked by peptide bond formed with α-carboxyl of L-lysine and ε-azyl from other L-lysine. The chemical formula for Epsilon Poly L Lysine (ε-Polylysine) as follows:

Epsilon Poly L Lysine molecular weight

3200~4500 g/mol (degree of polymerization = 25~35). Epsilon Poly L Lysine (ε-Polylysine) is a naturally produced, cationic homopolymer of 25-35 L-lysine(due to Streptomyces albulus fermentation.), which was connected by ε-amido bond compounded by a ε-amido of l-lysine and a a-carboxyl of another l-lysine.

Epsilon Poly L Lysine synthesis

α-Polylysine is a synthetic polymer, Epsilon Poly L Lysine (ε-Polylysine) fermentated from a fermentation process using Streptomyces albulus under aerobic conditions.

Epsilon Poly L Lysine solution

Usually pure Epsilon Poly L Lysine (ε-Polylysine) is dissolved to 5-10% Epsilon Poly L Lysine (ε-Polylysine) solution with cold boiled water or distilled water, then put it into the food and mix fully when in use.

Epsilon Poly L Lysine (ε-Polylysine) is hygroscopic, light yellow power. It is soluble in water, slightly soluble in ethanol, insoluble in organic solvent such as ethyl acetate, ether, ect.. The molecular of Epsilon Poly L Lysine (ε-Polylysine) is a straight chain polymer of lysine. It can decompose to lysine in human body, and can be completely digested to absorption by the human body. It doesn’t have any poisonous side effects and can be regarded as the source of amino acid lyisine.

Epsilon Poly L Lysine (ε-Polylysine) can inhibit the growth of Gram(+) and Gram(-) bacteria , yeast, mould, virus and ect, especially inhibit the Gram(-) bacteria which can’t be easily inhibited by other preservatives, such as E.coli, Salmonella, and also can inhibit the thermophilics, such as B,stearothermophilus. Its activity is not affected by PH, and stable when heated (120℃ for 20min). Therefore, it can be sterilized along with the raw material. Epsilon Poly L Lysine (ε-Polylysine) is a food preservative which is highly efficient, safe, and has no side-effects.

Physicochemical Property

Epsilon Poly L Lysine (ε-Polylysine) is a a linear polymer of lysine, it is a light yellow powder and is hygroscopic with a slightly bitter taste. The antibacterial activity is not affected by the pH value and stable to heat (120 ℃, 20min), and can inhibit the heat-resistant bacteria. As this result it can be added before heat treatment.

However, Epsilon Poly L Lysine (ε-Polylysine) antibacterial activity may be reduced due to the binding with acidic polysaccharides, hydrochlorides, phosphates, copper ions or others. Epsilon Poly L Lysine (ε-Polylysine) antibacterial activity can be enhanced With hydrochloric acid, citric acid, malic acid, glycine and higher fatty glycerides. Epsilon Poly L Lysine (ε-Polylysine) with a molecular weight between 3600-4300 has the best antibacterial activity. When the molecular weight is less than 1300, Epsilon Poly L Lysine (ε-Polylysine) loses its antibacterial activity. Since Epsilon Poly L Lysine (ε-Polylysine) is a mixture, there is no fixed melting point, and softening decomposition starts at 250 ° C or higher. Epsilon Poly L Lysine (ε-Polylysine) dissolved in water, slightly soluble in ethanol.

Biological Property

(1) Broad antibacterial spectrum: Epsilon Poly L Lysine (ε-Polylysine) has obvious inhibition to gram-positive bacteria and Gram-negative bacteria, yeast, mould, virus and many other microorganism.

(2) Good thermal stability: in high temperature (80℃-60 min, 100℃-30 min and 120℃-20 min), it does not decompose, not inactivation, suitable for cooked food.

(3) Good water-solubility: good water-solubility, it can spread evenly in food; Colorless, odorless and nature, do not affect food flavor.

(4) Wide PH range:Epsilon Poly L Lysine (ε-Polylysine) has broad antibacterial spectrum, the range of PH is between 2 and 9, most common food preservatives have poor antibacterial activity effects in neutral and alkaline conditions.

(5) Strong Blends: it has synergistic effect when blending with potassium sorbet, citric acid, malic acid, glycine and Nisin.

(6) High safety: After eating, it is not accumulated inside human bodies, partly break down into essential amino acid lysine for human body, L–lysine, Non-toxic, known as the “pharmaceutical & nutrient preservatives”

(7) Health function: Recent studies show that Epsilon Poly L Lysine (ε-Polylysine) can inhibit the enzyme activity and the fat absorption in the small intestine ester, has curative effect against with obesity.